Here, the olive leaf extract (OLE) rich in polyphenols was employed as a prebiotic agent, together with Lactobacillus reuteri and Bacillus clausii, to develop synbiotics. The prebiotic effect of olive leaf extract on the probiotic strains was tested at concentrations of 0, 50, 100, 400, and 1000 mu g mL(-1) , and also 20 and 40 mg mL(-1) . Olive leaf extract at 40 mg mL(-1) showed the best prebiotic activity on L. reuteri and B. clausii. A basal diet and two experimental synbiotic-containing diets were prepared. The synbiotic diets were manufactured by adding to the basal diet 5 x 10(6) CFU g(-1) L. reuteri + 5 x 10(6) CFU g(-1) B. clausii + 0.25 mg g(-1) OLE and 1 x 10(7) CFU g(-1) L. reuteri + 1 x 10(7) CFU g(-1) B. clausii + 0.25 mg g(-1) OLE. The diets were administered to the freshwater crayfish Astacus astacus (1.35 +/- 0.04 g) in an 84-day feeding trial. The diet containing 5 x 10(6) CFU g(-1) L. reuteri + 5 x 10(6) CFU g(-1) B. clausii + 0.25 mg g(-1) OLE significantly improved (p < 0.05) final weight, specific growth rate, body condition, and survival rate. A significant growth of Enterobacteriaceae, which include strains with proven beneficial activities for intestinal health and general animal welfare, significantly increased in crayfish fed with synbiotics. The obtained results could be suitable for functional feed development in crayfish farming.

Benefits of Polyphenol-Based Synbiotics in Crustacean Diet

Sateriale, D;Facchiano, S;Forgione, G;De Cristofaro, GA;Pagliarulo, C;Paolucci, M
2023-01-01

Abstract

Here, the olive leaf extract (OLE) rich in polyphenols was employed as a prebiotic agent, together with Lactobacillus reuteri and Bacillus clausii, to develop synbiotics. The prebiotic effect of olive leaf extract on the probiotic strains was tested at concentrations of 0, 50, 100, 400, and 1000 mu g mL(-1) , and also 20 and 40 mg mL(-1) . Olive leaf extract at 40 mg mL(-1) showed the best prebiotic activity on L. reuteri and B. clausii. A basal diet and two experimental synbiotic-containing diets were prepared. The synbiotic diets were manufactured by adding to the basal diet 5 x 10(6) CFU g(-1) L. reuteri + 5 x 10(6) CFU g(-1) B. clausii + 0.25 mg g(-1) OLE and 1 x 10(7) CFU g(-1) L. reuteri + 1 x 10(7) CFU g(-1) B. clausii + 0.25 mg g(-1) OLE. The diets were administered to the freshwater crayfish Astacus astacus (1.35 +/- 0.04 g) in an 84-day feeding trial. The diet containing 5 x 10(6) CFU g(-1) L. reuteri + 5 x 10(6) CFU g(-1) B. clausii + 0.25 mg g(-1) OLE significantly improved (p < 0.05) final weight, specific growth rate, body condition, and survival rate. A significant growth of Enterobacteriaceae, which include strains with proven beneficial activities for intestinal health and general animal welfare, significantly increased in crayfish fed with synbiotics. The obtained results could be suitable for functional feed development in crayfish farming.
2023
polyphenols
prebiotics
probiotics
synbiotics
crustaceans
Astacus astacus
microbiota
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12070/61282
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact