The relationship between intracellular Ca2+ ([Ca2+]i) regulation and programmed cell death is not well-defined; both increases and decreases in [Ca2+]i have been observed in cells undergoing apoptosis. We determined [Ca2+]i in cultured murine cortical neurons undergoing apoptosis after exposure to staurosporine or following oxygen-glucose deprivation in the presence of glutamate receptor antagonists. Neuronal [Ca2+]i was decreased 1 – 4 h after exposure to staurosporine (30 nM). A [Ca2+]i decrease was also observed 1 h after the end of the oxygen-glucose deprivation period when MK-801 and 6- cyano-7-nitroquinoxaline-2,3-dione (CNQX) were added to the bathing medium during the deprivation period. A similar decrease in [Ca2+]i produced by reducing extracellular Ca2+ or chelating intracellular Ca2+ was sufficient to induce neuronal apoptosis. Raising [Ca2+]i either by activating voltage-sensitive Ca2+ channels with () Bay K8644 or by application of low concentrations of kainate attenuated both staurosporine and oxygen-glucose deprivation-induced apoptosis.

Raising intracellular calcium attenuates neuronal apoptosis triggered by staurosporine or oxygen-glucose deprivation in the presence of glutamate receptor blockade

CANZONIERO L. M.;
2004

Abstract

The relationship between intracellular Ca2+ ([Ca2+]i) regulation and programmed cell death is not well-defined; both increases and decreases in [Ca2+]i have been observed in cells undergoing apoptosis. We determined [Ca2+]i in cultured murine cortical neurons undergoing apoptosis after exposure to staurosporine or following oxygen-glucose deprivation in the presence of glutamate receptor antagonists. Neuronal [Ca2+]i was decreased 1 – 4 h after exposure to staurosporine (30 nM). A [Ca2+]i decrease was also observed 1 h after the end of the oxygen-glucose deprivation period when MK-801 and 6- cyano-7-nitroquinoxaline-2,3-dione (CNQX) were added to the bathing medium during the deprivation period. A similar decrease in [Ca2+]i produced by reducing extracellular Ca2+ or chelating intracellular Ca2+ was sufficient to induce neuronal apoptosis. Raising [Ca2+]i either by activating voltage-sensitive Ca2+ channels with () Bay K8644 or by application of low concentrations of kainate attenuated both staurosporine and oxygen-glucose deprivation-induced apoptosis.
Calcium; Apoptosis; Staurosporine
File in questo prodotto:
File Dimensione Formato  
neurobiology disease.pdf

non disponibili

Licenza: Non specificato
Dimensione 507.81 kB
Formato Adobe PDF
507.81 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12070/5778
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 18
social impact