An androgen-binding activity has been identified in nuclear extracts of the testis of the frog, Rana esculenta. A single class of high affinity (I& = 2.5 * 0.6 x 10m9M), low-capacity binding sites was found. The binding was specific for androgens; 17@estradiol displaced [3H]testosterone with an ID,, of 0.1 PM. Cytosolic binding activity has a low affinity and a high capacity and lacks specificity. The seasonal fluctuations in binding capacity did not correlate with the androgen peaks in plasma and testes between February and June and in September; periods coinciding with the resumption of spermatogenesis and the development of spermatids, respectively. The present data strongly support androgenic control of intratesticular function in vertebrates generally.

Seasonal fluctuations of androgen-binding activity in the testis of the frog, Rana esculenta".

Paolucci M;
1992

Abstract

An androgen-binding activity has been identified in nuclear extracts of the testis of the frog, Rana esculenta. A single class of high affinity (I& = 2.5 * 0.6 x 10m9M), low-capacity binding sites was found. The binding was specific for androgens; 17@estradiol displaced [3H]testosterone with an ID,, of 0.1 PM. Cytosolic binding activity has a low affinity and a high capacity and lacks specificity. The seasonal fluctuations in binding capacity did not correlate with the androgen peaks in plasma and testes between February and June and in September; periods coinciding with the resumption of spermatogenesis and the development of spermatids, respectively. The present data strongly support androgenic control of intratesticular function in vertebrates generally.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.12070/4838
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