Besides its well recognized role in lipid and carbohydratemetabolisms, glycerol is involved in the regulation of cellularenergy homeostasis via glycerol-3-phosphate, a key metabolitein the translocation of reducing power across the mitochondrialinner membrane with mitochondrial glycerol-3-phosphate dehydrogenase.Here, we report a high rate of gluconeogenesisfrom glycerol and fatty acid oxidation in hepatocytes fromLou/C, a peculiar rat strain derived from Wistar, which is resistantto age- and diet-related obesity. This feature, associatedwith elevated cellular respiration and cytosolic ATP/ADP andNAD/NADH ratios, was linked to a high expression and activityof mitochondrial glycerol-3-phosphate dehydrogenase.Interestingly, this strain exhibited high expression and proteincontent of thyroid hormone receptor, whereas circulating thyroidhormone levels were slightly decreased and hepatic thyroidhormone carrier MCT-8 mRNA levels were not modified. Wepropose that an enhanced liver thyroid hormone receptor inLou/C may explain its unique resistance to obesity by increasingfatty acid oxidation and lowering liver oxidative phosphorylationstoichiometry at the translocation of reducing power intomitochondria.

High expression of thyroid hormone receptors and mitochondrial glycerol-3-phosphate dehydrogenase in the liver is linked to enhanced fatty acid oxidation in Lou/C rat strain resistant to obesity

MORENO M;GOGLIA F;
2009

Abstract

Besides its well recognized role in lipid and carbohydratemetabolisms, glycerol is involved in the regulation of cellularenergy homeostasis via glycerol-3-phosphate, a key metabolitein the translocation of reducing power across the mitochondrialinner membrane with mitochondrial glycerol-3-phosphate dehydrogenase.Here, we report a high rate of gluconeogenesisfrom glycerol and fatty acid oxidation in hepatocytes fromLou/C, a peculiar rat strain derived from Wistar, which is resistantto age- and diet-related obesity. This feature, associatedwith elevated cellular respiration and cytosolic ATP/ADP andNAD/NADH ratios, was linked to a high expression and activityof mitochondrial glycerol-3-phosphate dehydrogenase.Interestingly, this strain exhibited high expression and proteincontent of thyroid hormone receptor, whereas circulating thyroidhormone levels were slightly decreased and hepatic thyroidhormone carrier MCT-8 mRNA levels were not modified. Wepropose that an enhanced liver thyroid hormone receptor inLou/C may explain its unique resistance to obesity by increasingfatty acid oxidation and lowering liver oxidative phosphorylationstoichiometry at the translocation of reducing power intomitochondria.
THYROID HORMONE; MITOCHONDRIA; METABOLISM
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.12070/4064
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